Characterization and Fungicide Screening of a New Pathogen That Causes Leaf Spot on Rehmannia glutinosa

Outbreaks of leaf spot disease occurred in Rehmannia glutinosa fields Henan Province, China, 2019, with the incidence ranging from 20% to 40%. R. plants diseased leaves were collected, and 25 isolates obtained. Pathogenicity tests, morphological observations, phylogenetic analyses conducted identify pathogens, biological characteristics control agents pathogens studied. Five pathogenic fungi isolated. Three identified as Fusarium equiseti, which is a new pathogen causing disease; other two acuminatum. The mycelia F. equiseti grew fastest on Czapek medium, optimal temperature pH °C 10.0, respectively. 5 t o 35 °C, lethal was 55 °C. carbon nitrogen sources soluble starch peptone, Eight fungicides had inhibitory effects mycelial growth Prochloraz higher activities against acuminatum, EC50 values 0.139 mg·L−1 0.123 mg·L−1, These results provide useful information that will aid development management strategies diseases caused by